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In order to test the effect of crazy ant diet on interactions with fire ants,
aggression assays were again performed on July 31,2008, using 5 crazy ant workers and
5 small fire ant workers. Each crazy ant colony was used only once. Two fire ant
colonies were used twice, but each colony pair was unique. There were 6 aggression
assays performed for each of the 4 treatments for a total of 24 colony pairings.
Aggression and fighting data were recorded for 5 minutes, and mortality was recorded at
5 minutes, 1 hour, and 2 hours.

To determine the effect of diet on aggression score, fighting, and mortality of the
two species, 2-way ANOVA’s were performed with protein type, sugar level, and the
interaction of protein type and sugar level as factors. The following response variables
had a normal distribution without transformation: the average interaction score across all
time periods, the average number of crazy ants in engaged in fights, the average mɪmber
of fire ants involved in fights, and crazy ant mortality after 2 hours. The mortality of fire
ants after 2 hours was square root transformed prior to testing for a diet effect with an
ANOVA. Mortality after 2 hours was used because it had higher values and a more
normal distribution than 1-hour mortality, and in only 2 cases were all 5 of the ants from
a colony in a Petri dish dead (both occasions were fire ants). In order to test for a
difference between crazy ants and fire ants in average number of workers fighting and
mortality after 2 hours, the fire ant values were subtracted from the crazy ant values for
each response variable. A Wilcoxon signed rank test was used to test if the mean of the
difference in numbers of workers fighting was significantly different from zero, and an
ANOVA was used to determine if the mean of the difference in mortality after 2 hours
was affected by sugar level, protein type, or an interaction of the two variables. Also, a

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