Table of Contents
Abstract iii
Acknowledgements iv
ListofFigures vii
Chapter 1 Introduction
1.1 Scope and layout of this thesis 1
1.2 Lipid bilayer membranes 1
1.3 Membraneelectrostatics 4
1.4 AtomicForceMicroscopy 6
1.5 MembraneelectrostaticswithAFM 9
1.6 Electrostaticdoublelayerforceandchargeregulation 11
1.7 Chargeregulationatlipidinterfaces 12
Chapter 2 Quantitative Membranes electrostatics with AFM
2.1 Introduction 13
2.2 Materials and methods
2.2.1 Preparation of DOPC:DOPS supported 15
lipid membranes
2.2.2 Forcecurveacquisitionandanalysis 16
2.2.3 Tip charge density measurement 17
2.2.4 Tipradiusandspringconstantmeasurement 18
2.2.4 FiniteElementModeling 18
V