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662

Singh and Singh

Table I. Experimental Designs for Testing the Effect of Density on
Female Remating Frequency in Drosophila ananassae

Cross	Females	First males S	econd males
	(A) 2-h daily observation design
1	Bhutan	Bhutan	Bhutan
2	ca	DP	ca
3	ca	ca	DP
	(B) Continuous confinement design
4	ca	DP	ca
5	ca	ca	DP
6	ct rb	^y	ct rb
7	ct rb	ct rb	^y

for 2 h, the number of remated females in each vial was recorded, and re-
mated females were aspirated out. After 2 h of observation, the females and
males were separated by aspiration. Females were stored in groups of six
per vial and the same density was maintained by adding new once-mated fe-
males. Males were discarded. The procedure was repeated on 6 consecutive
mornings. The flies receiving a particular density treatment on 1 day always
received the same density treatment on following days. Two replicates were
run for wild-type (Bhutan) flies.

Table II. Results of the 2-h Daily Observation and Continuous Confinement Re-
mating Experiments at Different Densities in D. ananassae

Number of remated females at
different densities^a

Cross	2/V	6/V	12/V	24/V
A. 2-h daily observation design
1. Bhutan і × Bhutan × × Bhutan O'
Replicate I^b	5	7	4	3
Replicate II^b	6	2	4	5
2. ca ‘i' × DP × × ca ***	2	3	7	10
3. ca і' × ca × D DP **	3	5	9	11
B. Continuous confinement design
4. cat' × DP × × ca ****	7	10	15	19
5. cat' × ca × × DP ****	10	12	17	22
6. ctrb ^, ∣' × y × × ct rb ****	6	10	13	18
7. ct rb ‘i' × ct rb × × y *****	5	7	11	20

^a Twenty-four pairs were tested in each cross at each of the four densities.

^bVariation between remated and not-remated females nonsignificant (χ² test from
contingency table). Variation between remated and not-remated females: * P <
0.05; **P < 0.01; ***P < 0.001 (χ² test from contingency table).

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