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Figure 3.2 : (A) anti-HCNI antibody binding in photoreceptors. Labeling is present in the
inner segments of rods and cones. (B) HCN3 labeling also appears in photoreceptors and
other cells of the retina. (Al) western blot on mouse Brain (mB) and salamander retina (sR)
showing specific binding of the HCNl antibody to protein. Ihe resulting band is at 60 kDa
in the salamander retina, below the expected 104 kDa molecular weight for intact HCNl
protein. (Bl) western blot as in Al for the HCN3 antibody.




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