Table 1: Characteristics of migration of lymphocytes migrating through endothelial cell monolayers on clear substrates
Treatment/Cell |
Cells undergoing |
Cells undergoing |
Transit interval |
Migration velocity |
1 or more transits (%) |
more than 1 transit (%) |
(min) |
(μm∕min) | |
A. Migration of PBL |
through EC exposed to different cytokines - Static assay (n=4) | |||
TNF |
18.3 ± 8.6 |
6.6 ± 3.8 |
4.4 ± 0.9 |
6.4 ± 0.2 |
IFN |
25.9 ± 5.6 |
7.5 ± 3.9 |
4.4 ± 0.5 |
10.5 ± 1.4 |
TNF+IFN |
21.5 ± 4.3 |
9.6 ± 4.2 |
3.9 ± 0.2 |
8.9 ± 1.0 |
B. Different lymphocyte subsets migrating through |
EC treated with TNF+INF - |
Static assay (n=3) | ||
PBL |
17.5 ± 5.6 |
7.1 ± 3.4 |
3.6 ± 0.5 |
8.8 ± 2.4 |
CD3+ |
15.9 ± 6.0 |
5.2 ± 3.4 |
4.6 ± 0.8 |
6.1 ± 0.3 |
CD4+ |
19.2 ± 2.8 |
5.6 ± 2.9 |
3.8 ± 0.8 |
7.1 ± 1.6 |
C. Migration of PBL through EC exposed to different cytokines - Flow-based assay (n=4) | ||||
TNF |
23.9 ± 1.8 |
11.1 ± 3.8 |
3.7 ± 0.5 |
5.8 ± 0.5* |
IFN |
31.5 ± 4.0 |
13.7 ± 4.1 |
3.9 ± 0.5 |
9.6 ± 1.5 |
TNF+IFN |
32.5 ± 5.2 |
13.6 ± 7.9 |
4.4 ± 1.0 | |
D. Migration of PBL |
through EC on collagen gels - |
Static assay (n=3) | ||
TNF+IFN |
21.0 ± 2.5 |
7.8 ± 4.1 |
4.2 ± 0.4 |
5.4 ± 0.8 |
Individual lymphocytes were tracked over the 6min period and their location above or beneath the endothelium analysed every 10s. The
percentages of lymphocytes migrating through the monolayer once (1 transit) or migrating back and forth (>1 transit) were determined. The
average interval between transits for those cells that did move between compartments was also determined. The velocity of migration under the
monolayer was measured over a 5-minute period. Data are the mean ± SEM from n independent experiments. * ANOVA showed significant
effect of treatment on migration velocity, with TNF significantly different from IFN by Tukey test (both p<0.05).
More intriguing information
1. Commitment devices, opportunity windows, and institution building in Central Asia2. Sectoral specialisation in the EU a macroeconomic perspective
3. EXPANDING HIGHER EDUCATION IN THE U.K: FROM ‘SYSTEM SLOWDOWN’ TO ‘SYSTEM ACCELERATION’
4. Perceived Market Risks and Strategic Risk Management of Food Manufactures: Empirical Results from the German Brewing Industry
5. The name is absent
6. The name is absent
7. The name is absent
8. Two-Part Tax Controls for Forest Density and Rotation Time
9. The name is absent
10. Word searches: on the use of verbal and non-verbal resources during classroom talk