Direct observations of the kinetics of migrating T-cells suggest active retention by endothelial cells with continual bidirectional migration

Figure 7: Migration of leukocytes through EC and into collagen gels over time for A.
Neutrophils adherent to HUVEC treated with TNF for 4h; B. Lymphocytes adherent to
HUVEC treated with TNF+IFN for 24h. C. Lymphocytes adherent to HUVEC treated with
TNF+IFN for 24h, with or without addition of CXCL10 to the gel. Leukocytes were allowed
to settle on HUVEC for 10 min, non-adherent cells were washed off. In A and B, cells were
counted that were above the EC (phase bright), just below the EC (phase dark and spread) or
present within different regions as the microscope was focussed down into the gel. Data were
collected 0.25 ( I I ), 1 ( ∣ ∣ ), 3 ( I^ ) and 24h ( ∣ ∣ ) after initial addition of leukocytes, and
calculated as the percentage of the adherent cells. In C, the percentage of adherent
lymphocytes which had entered the gel was assessed with ( ■ ) or without ( □ ) CXCL10
added. Data are mean ± SEM from 3 experiments. In C, ** = p<0.01 compared to untreated
gel by paired t-test.

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