Deletion of a mycobacterial gene encoding a reductase leads to an altered cell wall containing β-oxo-mycolic acid analogues, and the accumulation of long-chain ketones related to mycolic acids



Table 2 MALDI-TOF/MS analysis of MAMEs isolated from different M. smegmatis strains. The masses indicated are those of Na-adducts and those
of predominant species are shown in bold.
α-epi is the isomer of α-mycolates.

Total Carbon number of rnycolic acid

Untreated____________________________________

Б2

64

74

75

76

77

78

79

____80

WT

ɑ'

952

980

ɑ

1118

1132

1146

1160

1174

1188

epoxy

1148

1162

1176

1190

1204

1218

NaBH4 treated

WT

o:'

952

980

α

1118

1132

1146

1160

1174

1188

epoxy

1162

1176

1190

1204

hydroxyl

1178

1206

1220

ΔMSMEG4722

ɑ'

952

980

a

1118

1146

1160

1188

a-epi

1160

1188

epoxy

1176

1190

hydroxyl

1177

ΔMSMEG4722-C

a

952

980

α

1118

1132

1146

1160

1174

1188

epoxy

1162

1176

1190

1204

hydroxyl

1178

1206

1220



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