Table 1
_______________________________Description____________________________ |
Reference/source__________________ | |
Plasmids | ||
pMV261 |
E. coli-Mycobacterium shuttle plasmid |
(Stover et al., 1991) |
pMV261-MSMEG4722 |
MSMEG4722 cloned in pMV261 |
This work |
pMV261-Rv2509 |
Rv2509 cloned in pMV261 |
This work |
p0004s |
Vector for cloning allelic-exchange |
Gift from T. Hsu and W.R.Jacobs Jr., |
p∆MSMEG4722 |
Derivative of p0004s designed for allelic |
This work |
Bacterial strains | ||
mc2155 |
Electroporation-proficient ept mutant of |
(Snapper et al., 1990) |
∆MSMEG4722 |
Deletion mutant of mc2155 in which MSMEG4722 is replaced by hyg |
This work |
∆MSMEG4722-C |
Complemented strain of ∆MSMEG4722 |
This work |
∆MSMEG4722-CRv |
Complemented strain of ∆MSMEG4722 |
This work |
Phages | ||
phAE159 |
Conditionally replicating shuttle phasmid |
(Bardarov et al., 2002) |
ph∆MSMEG4722 |
Derivative of phAE159 obtained by |
This work |
Plasmids, bacterial strains, and phages used in this study