Deletion of a mycobacterial gene encoding a reductase leads to an altered cell wall containing β-oxo-mycolic acid analogues, and the accumulation of long-chain ketones related to mycolic acids

Table 1

_______________________________Description____________________________		Reference/source__________________
Plasmids
pMV261	E. coli-Mycobacterium shuttle plasmid vector with hsp60 promoter and Kan^Rcassette (aph)	(Stover et al., 1991)
pMV261-MSMEG4722	MSMEG4722 cloned in pMV261	This work
pMV261-Rv2509	Rv2509 cloned in pMV261	This work
p0004s	Vector for cloning allelic-exchange substrates to be used for specialized transduction; contains λ phage cos site and Hyg^R cassette (hyg)	Gift from T. Hsu and W.R.Jacobs Jr., Albert Einstein College of Medicine, New York
p∆MSMEG4722	Derivative of p0004s designed for allelic exchange of M. smegmatis MSMEG4722	This work
Bacterial strains
mc²155	Electroporation-proficient ept mutant of M. smegmatis strain mc²6	(Snapper et al., 1990)
∆MSMEG4722	Deletion mutant of mc²155 in which MSMEG4722 is replaced by hyg	This work
∆MSMEG4722-C	Complemented strain of ∆MSMEG4722 containing pMV261-MSMEG4722	This work
∆MSMEG4722-CRv	Complemented strain of ∆MSMEG4722 containing pMV261-Rv2509	This work
Phages
phAE159	Conditionally replicating shuttle phasmid derived from the lytic mycobacteriophage TM4	(Bardarov et al., 2002)
ph∆MSMEG4722	Derivative of phAE159 obtained by cloning p∆MSMEG4722 into its unique Pac I site___________________________________	This work

Plasmids, bacterial strains, and phages used in this study